The venoms alone or preincubated with arjunolic acid(0.1–30 μg/mL) for 30min were
added to the solution,and the tubes were incubated for15min
at 37 1C. Hexadecytrimethylammonium bromide(0.666mL,2.5%)
was added to the tubes for absorbance readings at 400nm.Results
are expressed in percentage of venom activity vs. arjunolic acid
concentration.