The identification of chitinolytic bacteria showed that isolate 6.0 was Sphingobacterium and isolate 6.1 was Brevundimonas. The N-acetyl glucosamine and chitooligomers production by Sphingobacterium sp. 6.0 and Brevundimonas sp. 6.1 were studies. It was found that the chitin digestion in chitin medium composed of 1.0% chitin and 0.5% peptone with initial volume of 150 ml gave the maximum production of N-acetyl glucosamine and chitooligomers by cultivation on 200-rpm of shaking speed at 25o C for Sphingobacterium sp. 6.0. In the case of Brevundimonas sp. 6.1, the optimal temperature was shifted to 30o C. The alteration of temperature during cultivation showed some effects on the accumulation of N-acetyl glucosamine and the ratio of chitooligomers. The Nacetyl glucosamine accumulation was increased by the cultivation of Sphingobacterium sp. 6.0 and Brevundimonas sp. 6.1 at 25o C and 30o C, but the production of chitooligomers was minimized.