D1 protein is located in the reaction center of photosynthesis system II (PSII) where the water is splitted to oxygen, proton and electron. Oxygen can function as an inhibitor of hydrogenase enzyme which catalyzing formation of molecular hydrogen from proton and electron. Inhibition of D1 protein resulted in low level of molecular oxygen. This level of oxygen can be consumed by respiratory chain in mitochondria resuling in no inhibitor of hydrogenase in the stroma of chloroplast. The hydrogen production should be obtained in high yield.