Fig. 1. Relative quantification of VP2 gene expression assessed by real-time PCR. Total
mRNA was obtained from different tissues (head kidney, spleen, gut, and gill) at 1, 3, 5
and 15 days post-vaccination with pcDNA-VP2. The vaccine was incorporated into
commercial pellets and the fish were fed the DNA vaccine diet on three consecutive
days. The first day without feeding vaccine pellets was considered day 1 postvaccination.
The data are shown as the mean gene expression relative to the expression
of the endogenous control EF-1a (2DCt method). The error bars represent the St.
dev. of the mean (n ¼ 3).