Plant material was air dried at room temperature. The sample
was crushed and deposited in an Erlenmeyer flask that contained
methanol for the whole night, filtered, and the process repeated five
times. The resulting methanol extract was concentrated at reduced
pressure in a rotatory evaporator at 40 ◦C and 200 mb obtaining a
syrups methanol extract (645 g).
A portion of the methanol extract (410 g) was dissolved with distilled
water to a ratio 60/40 methanol/water, then this solution was
placed in a separator funnel, and a distribution became liquid/liquid
with 150 ml of n-hexane 20 times, then n-hexane phases combined
and concentrated under reduced pressure. An identical process was
repeated with ethyl acetate and finally obtained a residual mixture
methanol/water.