2.4. Larvicidal bioassay
The larvicidal bioassay was assessed by using standard[15].
The different solvent leaf extracts prepared from A. paniculata
and A. lineata were subjected to dose-response bioassay for
larvicidal activity against the larvae of Ae. aegypti and Cx.
quinquefasciatus. In addition to the larvicidal activity of the
plants A. paniculata and A. lineata extracts individually,
both the extracts are mixed in an equal proportion (1:1),
and mixed well for both the extracts to interact well, and
the larvicidal assay was performed. The stock solution
of the aqueous extracts are mixed in 1:1 ratio to give
100% concentration stock, which is diluted to required
concentrations for combination extract. Batches of 30 fourth
instar larvae were transferred to a small disposable test
cups, each containing 200 mL, of water in the cups to obtain
the desired target dosage (concentrations ranging from 50
to 200 ppm), starting with the lowest concentrations.