Temperature and pH optima, stability in different temperature and pH
The best pH for activity of the purified xylanases was determined by
assaying enzymatic reactions inMcIlvaine buffer adjusted to various pH
between 3.0 and 8.0, with 0.5 unit intervals at 50°C. For the optimal
temperature, enzymatic reactions were carried out with the purified
enzymes in 0.05 M sodium acetate buffer pH 5.5 at temperatures from
20 to 60°C, with 5°C intervals