The microalga used in this work was S. obliquus from the
Coimbra University Algae Collection (Portugal). The pre-inoculum
was grown in 0.8 L of Bristol medium (pH 7) [18] in a 1 L glass bubble column reactor, agitated by filtered compressed air, at 25 C
and under artificial light. One week after inoculation, the culture
was transferred to a 50 L bubble column photobioreactor (PBR)
with the same medium, outdoor. One week later, the entire volume
was used as an inoculum for a 600 L outdoor raceway open pond.
After growth, 400 L of the culture were transferred to a 5000 L outdoor raceway pond and grown during two months. Pond agitation
was promoted by paddle wheels, with no additional artificial light,