A specific PCR assay was successfully developed for detection and quantification
of commercial Influenza A virus. However, no viral RNA was found in the air
samples from Nationaltheatret subway station. Inhibition of the PCR reaction
was observed, and hence further investigation regarding inhibition is needed in
order to rule out false negative results. Furthermore, longer sampling times
should be tested in order to ensure collection of detectable concentrations of
virus. The efficiency of the SASS3100 air sampler in collecting virus could be
tested by use of other air samplers in parallel to SASS3100 in hospitals or other
indoor locations known to contain airborne Influenza virus.