mold in culture flasks containing 4oo ml of medium per flask. We have used i8-1
pyrex bottles containing 13 1 of medium each. The length of time required for the
optimal production of the nucleoside was of the same order of magnitude reported
for the smaller culture volumes 1.
All slant cultures were maintained on oatmeal agar prepared as follows: 30 g of
quick-cooking rolled oats and 600 ml water were cooked for 30 to 45 min in a double
boiler. This material was then strained through cheese cloth. 12o ml of this extract
were autoclaved for 30 min with 280 ml water and 12 g agar. (io-ml portions of this
were dispensed into test tubes, autoclaved, and slanted.) Cultures were transferred
about every two weeks.