2.3. The Effect of Inducers on the Tannase Activity
The fungus Aspergillus sp. GM4 was initially grown at 30˚C for 48 h (100 rpm) in the modified Adams medium containing 2% (w/v) glucose as a carbon source. Then, the mycelia mass was separated by vacuum filtration, washed with sterilized distilled water, transferred in aseptic conditions to the modified Adams medium with the appropriate inducers (w/v) (1% and 2% tannic acid; 1% green tea; 1% methyl gallate; 1% gallic acid) and incubated at 30˚C for 48 h and 100 rpm. The mycelia mass was also transferred to a flask containing 1% (w/v) glucose and to a control flask without a carbon source. After the incubation time, the tannase activity was meas- ured, and the induction ratio was calculated by dividing the induced tannase activity by the basal tannase activity from the medium containing glucose as the sole carbon source [13].