Application to Real Samples. The optimized and
validated analytical methodology was applied to the analysis
of 12 squid samples of Loligo gahi species and to 6 hemolymph
samples obtained from Octopus vulgaris harvested live from the
Northwest Atlantic Portuguese coast. L. gahi is widely
distributed along the Pacific coast of South America. It is the
second most important loliginid squid in commercial fisheries
worldwide.39 A small part of the total catch is consumed in
Europe, predominantly in Spain.2 With regard to the selected
octopus, it corresponds to the major species consumed
worldwide40 and was recently proposed as a sentinel species
for the presence of contaminants in coastal waters.13