The bone was first weighed in air and then weighed by submerging it in distilled water using the integral weigh below hook facility. The volume is calculated by subtracting the wet weight from the dry weight, and its density was determined by dividing the dry weight by the volume (Giancoli, 1998). The bone was placed in an oven at 100 °C for 16 h to determine the dry matter weight. The sample was then ashed at 650 °C in a muffle furnace and the ash was digested in aqua regia (HCl/HNO3 mixture).