In 1934–1935, Gautheret together with White and Nobercourt,
developed the first “true” plant tissue culture since it involved the
establishment of cambial tissue from Acer pseudoplatanus L. in a
solidified medium combining knop’s solution, glucose, cysteine,
IAA and vitamins that lead to the differentiation of the tissues,
obtaining anendless callus culturesproliferation(Gautheret, 1939).
From that moment, different plants, organs and tissues but also
different combinations of nutrient solutions were being tested in
numerous trials. The decades between 1940 and 1960 are pointed
out as one of the most important period for the development of the
great majority of the techniques of plant tissue culture still used
today. During that time many discoveries were made mainly in the
field of plant growth regulators: kinetin was found in 1955 as a cell
division hormone, leading Skoog and Miller in 1957 to arise the
concept of hormonal control for organ formation by adjusting the
concentration of auxins and cytokines in the medium (Skoog and
Miller, 1957).