Iron (Fe) content
The iron contents in rice were determined
using atomic absorption spectrophotometer-AAS
(100 Analyzer, Perkin Elmer, Germany). The iron
detection limit of AAS used in this study was 0.039
mg/kg. Dehusked rice flour samples (2–4 g) were
wet-acid digested with nitric acid and solubilized
with 10 mL of deionized water (Lestienne et al.,
2005). The absorbance of solubilized samples was
measured. The absorbance of iron solutions (0.5, 1,
2 and 4 g/mg) were measured in the same manner
as the samples, and standard curve was created. The
regression equation obtained was y = 0.0358x-0.0028
(R2
= 0.9993).