The method for the quantitative determination of DHEA-S is a competitive-binding chemiluminescence immunoassay.
A specific mouse monoclonal antibody to DHEA-S is coated on the magnetic particles (solid phase, catcher antibody);
DHEA-S is linked to an isoluminol derivative to form a conjugate.
During the incubation, DHEA-S present in calibrators, samples or controls competes with the isoluminol-DHEA-S conjugate
for a fixed and limited amount of monoclonal antibody to DHEA-S bound to the solid phase. After incubation, the unbound
DHEA-S is removed from DHEA-S bound to magnetic particles by aspiration of the reaction mixture and a subsequent wash
cycle.