Morphology Study: Scanning electron microscopy (SEM,
S-4800) and confocal laser scanning microscope (ZEISS, LSM710)
were used to study the microstructure of the SF scaffold. The
micrographs of the 3D structure were obtained. We used Nano
Measurer Software (version 1.2) to determine the pore sizes by
randomly measuring 15 pores of the CLSM micrographs.