Materials &Methods
Isolation of endophytic fungi
Healthy and mature Centella asiatica were collected from different places in and around
Namakkal District, Tamil Nadu and was used for the isolation of endophytic fungi. The plant was
taxonomically identified and authenticated by Botanical Survey of India, Tamilnadu Agricultural
University, Coimbatore. The voucher specimen was deposited there with register number
BSI/SRC/5/23/2011-12/Tech.-932.The fresh plant samples were taken to the laboratory and treated
within few hours. Fresh materials were used for isolation of endophytes. A modified procedure was
followed for surface sterilization and isolation of endophytic fungi as described by Schulz et al.
1993. Colonization Frequency (CF) was calculated as described by Suryanarayanan et al. 2003.
Number of segments colonized by fung