Lipase (50 mg) from Pseudomona Cepacia was dissolved in 0.5 ml
of 0.01 M phosphate buffer at pH 7.0 (this was the optimum pH for
the lipases as reported by the vendors and other workers [5–8]). The
enzyme solution was immediately frozen at −20 ◦C and lyophilised
for 24 h [26]. These were referred to as “pH-tuned” enzyme preparations.