Hydrolyzed Bermuda grass (Cyanidon dactylon) and Jambul seed (Syzygium cumini),were used as carbon sources for the production of Polyhydroxyalkanoate (PHA) from soilmicrobial isolates. The efficiency of selected isolate for PHA production utilizing thehydrolyzed substrate as carbon source was compared with Ralstonia eutropha
(referencestrain) using the same production medium. The best isolate SP-Y1 and Ralstonia eutropha
were able to accumulate 26.76% and 28.97% of their dry cell weight when hydrolyzed grasswas used as substrate and PHA accumulation increased to 41.7% and 42.2% when hydrolyzedseed was used as a sole carbon source