The supernatant was 88 K.-H. Lin et al. / Scientia Horticulturae 150 (2013) 86–91 applied to determinate the absorbances of chl a, chl b, and car in acetone, as measured with a spectrophotometer (U-2000, Hitachi, Tokyo, Japan), at respective wavelengths of 663, 645, and 470 nm.