2.5. Skin Irritation Studies. In another study, the potential
systemic toxicity and local irritative potential of HCA-SX
were evaluated using Albino rabbits. HCA-SX administration at a single dose of 500 mg/site was directly applied to
shaved intact skin to assess the local dermal irritative potential. Each animal received a single, 4 h semioccluded exposure
and application sites were evaluated at approximately 30–
60 min and 24, 48, and 72 h after patch removal. Minimal
irritation was noted in this study. Very slight erythema on
a single animal was noted at the beginning of observation,
but completely subsided by the end of day 1. All dose
sites were stained yellow. No gross toxicological pathology
(except for reddened application sites and accessory spleen
for two rabbits each, and single occurrence of pale kidneys,
mottled lungs, and hair loss for one rabbit each) was foundon autopsy. HCA-SX was classified as nonirritating, as the
primary irritation index was calculated to be 0