The purity of PCR products was checked by electrophoresis in 1% agarose gel followed by staining with GelRed dye (Biotium, Hayward, CA, USA) and used for RFLP and sequence analyses.
The purity of PCR products was checked by electrophoresis in 1% agarose gel followed by staining with GelRed dye (Biotium, Hayward, CA, USA) and used for RFLP and sequence analyses.
The purity of PCR products was checked by electrophoresis in 1% agarose gel followed by staining with GelRed dye (Biotium, Hayward, CA, USA) and used for RFLP and sequence analyses.