2.7. Spore suspension preparation
According to Abo-State [20], the fungal isolates were inoculated using 100 mL of PDA medium in 250 mL Erlenmeyer
flasks. The inoculated media were incubated at 28 C for 7 d,
and then the spores were collected by adding 30 mL of sterile
saline containing 0.1% (v/v) Tween-80. The spore suspension
of each flask was collected in a new sterile flask as stock for
inoculation. The spore suspension count was 4 • 107.