. For chemotaxonomic analysis, freeze-dried cells were obtained from a culture grown in ISP 4 broth on a shaking incubator at 120 r.p.m. and 28 uC for 14 days. Identification of the diaminopimelic acid in the cell wall and analysis of whole-cell sugars were performed as described by Lechevalier & Lechevalier (1970, 1980) and Staneck & Roberts (1974), respectively. Polar lipids were extracted and detected according to the method of Minnikin et al. (1984). Menaquinones were extracted as described by Collins (1985) and were separated by HPLC. The G+C content of the genomic DNA was determined
by the HPLC method according to Mesbah et al. (1989).The major cellular fatty acid was iso-C16: 0 (see Table S1 available in IJSEM Online). The cell-wall peptidoglycan contained LL-A2pm, and glutamine, alanine and glycine. Whole-cell hydrolysates contained predominantly xylose and arabinose. The polar lipid pattern consisted of phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylserine and three unknown phospholipids (Fig. S1).
The predominant menaquinones were MK-9(H6) (51.22%), MK-9(H8) (44.4 %) and MK-9(H4) (4.37%). The G+C content of the genomic DNA of strain BR-34T was 72.8 mol%.
. For chemotaxonomic analysis, freeze-dried cells were obtained from a culture grown in ISP 4 broth on a shaking incubator at 120 r.p.m. and 28 uC for 14 days. Identification of the diaminopimelic acid in the cell wall and analysis of whole-cell sugars were performed as described by Lechevalier & Lechevalier (1970, 1980) and Staneck & Roberts (1974), respectively. Polar lipids were extracted and detected according to the method of Minnikin et al. (1984). Menaquinones were extracted as described by Collins (1985) and were separated by HPLC. The G+C content of the genomic DNA was determinedby the HPLC method according to Mesbah et al. (1989).The major cellular fatty acid was iso-C16: 0 (see Table S1 available in IJSEM Online). The cell-wall peptidoglycan contained LL-A2pm, and glutamine, alanine and glycine. Whole-cell hydrolysates contained predominantly xylose and arabinose. The polar lipid pattern consisted of phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylserine and three unknown phospholipids (Fig. S1).The predominant menaquinones were MK-9(H6) (51.22%), MK-9(H8) (44.4 %) and MK-9(H4) (4.37%). The G+C content of the genomic DNA of strain BR-34T was 72.8 mol%.
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