The isolation procedure may be quite complicated
depending on the constituent to be analyzed.
• For example, analysis of triglyceride bound fatty acids in a
food will involve the extraction of lipids (free fatty aicds,
mono-, di-, and triglycerides, sterols, fat-soluble vitamins,
etc.) from the food by solvent extraction, and the isolation
of triglyceride fraction by adsorption chromatography.
• The isolated triglycerides would have to be hydrolyzed to
give fatty acids and subsequently to form esters to
improve gas chromatography properties.