2.8. Assay of Superoxide Dismutase Activity
The uterine tissue was taken in 4 mL of chilled Tris buffer (50 mM pH 8.2) and was homogenized at 13,000 rpm (3 cycles of 30 sec each), using a Polytron homogenizer. The homogenate was treated with 1 mL of 0.1% Triton X 100