Stevioside is a natural sweetener extracted from leaves of Stevia rebaudiana Bertoni, which is commercially
produced by conventional (chemical/physical) processes. This article gives an overview of the stevioside
structure, various analysis technique, new technologies required and the advances achieved in recent years.
An enzymatic process is established, by which the maximum efficacy and benefit of the process can be
achieved. The efficiency of the enzymatic process is quite comparable to that of other physical and chemical
methods. Finally, we believe that in the future, the enzyme-based extraction will ensure more cost-effective
availability of stevioside, thus assisting in the development of more food-based applications.