Twenty lactic acid bacteria were screened for the abilities of rat intestinal α-glucosidase inhibition,short-chain fatty acids production,utilization of prebiotics as well as gastrointestinal tract tolerance. Nine strains inhibited α-glucosidase, four of which, Lactobacillus acidophilus CCFM6,Lactobacillus plantarum CCFM47,CCFM232 and Lactobacillus rhamnosus GG (LGG) were tolerant to simulated gastrointestinal juices with survival rates up to 60% following simulateddigestion.Whengrownonsoybeanoligosaccharides(SBOs),CCFM47producedpropionic (39.9 mM) and butyric (3.5 mM) acids while strain CCFM6 produced 17.2 mM propionic acid. Strains CCFM6 and CCFM47 were further tested for their viability and survival in set yoghurt supplemented with SBOs. SBOs enriched yoghurt improved probiotic survival in the simulated gastric juice and significantly improved α-glucosidase inhibition (P < 0.05).Thus strains CCFM47 and CCFM6 can be used as probiotics with antihyperglycaemic potential in the formulation of functional foods such as yoghurt.