The single chain recombinant eFSH was constructed using the cDNAs encoding eFSH alpha (G.R.
Bousfield, Wichita State U; Dalpathado et al., 2006) and eFSH beta (K. Shiota, U Tokyo; Saneyoshi et
al., 2001). The recombinant eFSH was cloned using Chinese hamster ovary (CHO) cells and methods
carried out for reLH (Jablonka-Shariff et al., 2007). The carboxyl terminal peptide of the eCG/eLH beta
was used as a spacer to fuse the amino terminus of the alpha subunit (without its signal peptide) with
the carboxyl end of the FSH beta subunit (Fig. 1). The reFSHwas demonstrated to be biologically active
in vitro (Roser et al., 2004).
The single chain recombinant eFSH was constructed using the cDNAs encoding eFSH alpha (G.R.Bousfield, Wichita State U; Dalpathado et al., 2006) and eFSH beta (K. Shiota, U Tokyo; Saneyoshi etal., 2001). The recombinant eFSH was cloned using Chinese hamster ovary (CHO) cells and methodscarried out for reLH (Jablonka-Shariff et al., 2007). The carboxyl terminal peptide of the eCG/eLH betawas used as a spacer to fuse the amino terminus of the alpha subunit (without its signal peptide) withthe carboxyl end of the FSH beta subunit (Fig. 1). The reFSHwas demonstrated to be biologically activein vitro (Roser et al., 2004).
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