Fig. 1a and b shows the production of ethanol, residual reducing sugars and ethanol yields for different types of root hydrolysates used without concentration and after concentration, respectively. Time course of batch fermentation (Fig. 1a) showed that the ethanol production started at the end of exponential phase (approximately 6–8 h). Initial reducing sugars and glucose content of the normal root hydrolysate (without treatment) was found to be 43 g/l and 33.7 g/l, respectively. Ethanol concentration and yield of the normal root hydrolysate after 84 h of the anaerobic fermentation was found to be 16.15 g/l and 0.38 g/g, respectively. No significant increase was found after 84h of the fermentation in ethanol concentration. Yeast grows in the anaerobic conditions as long as there is oxygen present in the medium, after that ethanol production stage initiates. Maximum ethanol concentration and yield using standard production medium (control) was found to be 14.64 g/l and 0.49 g/g, respectively after 60 h of fermentation with no further significant increase even after increasing the fermentation time. Protein content of the C. forskohlii roots (6.5%) may be sufficient for the growth of the S. cerevisiae as well as ethanol production.