system,HSA,can also be conveniently estimated by this method
and the resolved distribution diagram is shown in Fig.4 D. Based on
the calculation of the absolute concentration of all species in an
equilibrium state,the apparent binding constant at 298.15 K can be
estimated to be 8.4108 M1, which correlates well with earlier
reports [49]. As can beclearly seen in Fig.4 C, there is almost no
difference between the true spectra and resolved spectra in the case
of AuNPs with the similarity coefficient of 0.9999,further giving
evidence for the absence of rotational ambiguity in ther esolution
process.Inaddition,the SPR band of the AuNPs–HSA conjugate,
relative to that of AuNPs,broadens,intensifies and red-shifts from
519nm to 523nm and this conjugate has a molar extinction
coefficient of 2.6108 M1 cm1 at peak wavelength, 1.1-fold
larger than that of AuNPs (Fig.4C). Fig.4D displays that the absolute
concentrations of HSA are very low before the binding process
reaches a steady state,and then they increase almost linearly with
the increasing concentrations of HSA
system,HSA,can also be conveniently estimated by this methodand the resolved distribution diagram is shown in Fig.4 D. Based onthe calculation of the absolute concentration of all species in anequilibrium state,the apparent binding constant at 298.15 K can beestimated to be 8.4108 M1, which correlates well with earlierreports [49]. As can beclearly seen in Fig.4 C, there is almost nodifference between the true spectra and resolved spectra in the caseof AuNPs with the similarity coefficient of 0.9999,further givingevidence for the absence of rotational ambiguity in ther esolutionprocess.Inaddition,the SPR band of the AuNPs–HSA conjugate,relative to that of AuNPs,broadens,intensifies and red-shifts from519nm to 523nm and this conjugate has a molar extinctioncoefficient of 2.6108 M1 cm1 at peak wavelength, 1.1-foldlarger than that of AuNPs (Fig.4C). Fig.4D displays that the absoluteconcentrations of HSA are very low before the binding processreaches a steady state,and then they increase almost linearly withthe increasing concentrations of HSA
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