Extraction of B. racemosa seed in methanol solvent. Fruits of B. racemosa were pared
and the seed was thin-sliced using a knife, and sliced seeds were dried under indirect
sunlight for 5 days. The dried slice seed was blended to make powders with a sieve size
of 40 meshes. The powders were weighed as much as 100 g, and then put into a jar (5 L
in volume). A total of one liter of absolute methanol was poured slowly into the jar and
stirred homogeneously, and then the mixture was left for 24 hours. The mixture was
separated with Whatman #1 paper. The filtrate was evaporated using an evaporator
vacuum to obtain viscous crude extracts. The extracts were dried in a desiccator for eight
hours.
Extraction of B. racemosa seed in water solvent. A total of 200 g of seeds were thinsliced,
and then the slices were blended to make pulp. The pulp was boiled within 100 mL
water for 15 minutes, the decoction was filtered using Whatman #1 paper. The filtrate
was evaporated through an evaporator vacuum to obtain viscous crude extracts. The
specific gravity of condensed extracts was determined and then they were stored in a
refrigerator for two days.