In this study, dimethyl sulfoxide performed similar to ethylene glycol and propylene glycol. Ethylene glycol resulted better than dimethyl sulfoxide and propylene glycol. Even though glycerol resulted in highest sperm motility rates than the other cryoprotectants, when it was combined with ethylene glycol the sperm motility rate decreased. Ethylene glycol has a high penetrating ability into the cell, which may cause damage to the cell membrane. Moreover, there was a significant difference on sperm motility rates of fresh (94.7 ± 2.6%) and frozen/thawed semen. These findings are in line with the results reported by Watson [21] and Seshoka et al. [7], who indicated that regardless of the concentration and cryoprotectant used for cryopreservation, the freezing-thawing process reduced the Nguni total sperm motility rates