To determine optimal pretreatment methods for ethanol
production from cassava pulp, hydrothermal reaction with and
without dilute sulfuric acid was examined. The experimental
procedure is summarized in Fig. 1. First, to test the effects of
a combination of hydrothermal reaction (120–180 C for 60 min)
and addition of H2SO4 (0.1% and 2%), glucose yield in the pulp
hydrolysates was measured after enzyme treatments of the cassava
pulp (Fig. 1).