An additional test was performed for the LAB strains that had both antibacterial activities to V. harveyi and V. parahaemolyticus ATCC 17802. The supernatant was isolated from the LAB culture grown in 200 mL MRS broth for 24 h at 37 °C and adjusted to pH 6.5 with NaOH (100 mmol/L). It was then dissolved in 20 mL distilled water after
lyophilization and stored at −20 °C until use. The antibacterial activity assay was the same as with the cell-free culture supernatant.