where N is the number of theoretical plates;
Hp is the height of each plate;
tR, tR1 and tR2 are the retention times of each compound;
tRsolv: is the dead time;
w is the width of the peak at the baseline;
W0.05 is the width of the peak at 5% of the peak height;
W1/2 (1) and W1/2 (2)are the width of the peak at half-height for each compound;
L is the length of the column;
k’ is the retention factor;
T is the tailing factor;
f is the distance between the perpendicular dropped from the peak maximum and the leading edge of the peak at 5% of the peak height; and R is the resolution factor.