MS medium supplemented with different concentrations of 2,4-D (0.5-4.0 mg/l) was used to study the effect
of 2,4-D on callus induction from leaves of in vitro grown plantlets of Gardenia latifolia. White friable or creamy
white colored friable calli obtained on all the concentrations of 2,4-D after 8 weeks of culture. The high frequency of
callus induction was observed on MS medium supplemented with 3.0 mg/l 2,4-D (80±0.10%). The initiated primary
callus was subcultured on to MS medium with various combinations of BA (1.0-2.0 mg/l) and IAA (0.1 mg/l).
Embryogenic callus formation observed on MS medium with various combinations of BA (1.0-2.0 mg/l), Kn (2.0
mg/l) and IAA (0.1 mg/l). Histological analysis revealed the developmental pattern of globular, heart shaped
embroys. The protocol developed in this study, will be used to obtain embryogenic callus from leaf explants.
Keywords: Callus induction, Gardenia latifolia, Somatic embryos