Anticoagulant
Blood is collected from the wing veins of three chickens as described in Section 7. It is mixed with an anticoagulant. Two anticoagulants are commonly used and easy to prepare:
Acid citrate dextrose (ACD) solution. Allow 1 part ACD to 3 parts blood.
Alsever's solution. Allow 1 part Alsever's to 1 part blood.
Recipes for ACD and Alsever's solution are provided in Appendix 3.
Two techniques for collection of blood into anticoagulant