A quantitative real-time PCR using a primer pair CM2946F/CM3160R was developed for specific detection
and quantification of Cordyceps militaris from soil. Standard curves were obtained for genomic DNA and
DNA extracts from autoclaved soil with a certain dose of C. militaris suspension. C. militaris was detected
from two forest soil samples out of ten that were collected when fruit bodies of C. militaris were found.
This method seemed effective in detection of C. militaris in the soil and useful for rapid and reliable quantification
of C. militaris in different ecosystems