For each generation, another 100-150 protocorms or PLBs obtained by induction of each of diploid, triploid and tetraploid of Phal. aphrodite subsp. formosana, and of diploid Phalaenopsis bellina are selected and induced in the followingexperimental steps so as to regenerate the plants. Firstly, the protocorms or PLBs are subcultured in a T2 inducing medium provided in a Petri dish. Subsequently, the Petri dishes are placed in a culture room where the temperature is within25.+-.2.degree. C. the illuminance is 2,000 1.times., and the illuminating time is 16 hrs per day. After culturing for 3 to 5 months, regenerated plantlets grown with 3-4 leaves are obtained for further studies.
For each generation, another 100-150 protocorms or PLBs obtained by induction of each of diploid, triploid and tetraploid of Phal. aphrodite subsp. formosana, and of diploid Phalaenopsis bellina are selected and induced in the followingexperimental steps so as to regenerate the plants. Firstly, the protocorms or PLBs are subcultured in a T2 inducing medium provided in a Petri dish. Subsequently, the Petri dishes are placed in a culture room where the temperature is within25.+-.2.degree. C. the illuminance is 2,000 1.times., and the illuminating time is 16 hrs per day. After culturing for 3 to 5 months, regenerated plantlets grown with 3-4 leaves are obtained for further studies.
การแปล กรุณารอสักครู่..