Induction of diabetes
Diabetes simulating insulin dependence (type
1 diabetes) was induced by intraperitoneal injection
of streptozotocin (STZ, 55 mg/kg body weight,
Sigma, USA) to adult male Sprague-Dawley rats.
After two weeks, rats with a nonfasting blood
glucose over 200 mg/dl were included in the
experiment (Rakeiten et al., 1963). Non insulin
dependent diabetes (type 2 diabetes) was induced
by intraperitoneal injection of STZ 90 mg/kg body
weight to 48-hour old pups of Sprague-Dawley rats
(Bonner-Weir et al., 1981). At 8 weeks old, the
rats with nonfasting blood glucose more than 200
mg/dl were used in the study.