Introduction
Taihu Lake, the third largest freshwater lake in China, has
suffered from frequent algae blooms for several years. Every
year more than 0.2 million tons of algae covering a quarter of
the lake with 10 cm thickness, are harvested from Taihu Lake
[1]. Algae blooms severely pollute the environment and pose
risk to the safety of potable water within the area. However,
due to the rapid growth of algae, the huge amount of algal
biomass can be potentially used as feedstock for sustainable
biofuels production. There are a lot of studies on biofuels
production from algal biomass (e.g., Chlorella vulgaris ESP6,
Arthrospira platensis, and Chlamydomonas reinhardtii) [2e4].
However, studies on the use of Taihu algae blooms are
limited. For instance, algae bloom freshly collected alongside
Taihu Lake (moisture content: 95%e98%; carbon, nitrogen,
phosphate, and ash contents: approximately 440, 68, 6.7, and
82 mg/g, respectively) pretreated with alkaline yields an energy
of 1.1 kJ of hydrogen per gram of total volatile solid (TVS)
through dark-fermentation [5]. In another study, acid stress
on anaerobic sludge was induced using a stress medium
containing 12.5 g/L of acetate for 48 h with appropriate
shaking. The pH was adjusted to 5.5 and the sludge was
washed twice with tap water for removing residual acids
before initiating the fermentation process. Acid stress on
sludge also improves hydrogen energy recovery up to 1.4 kJ/gTVS
[6]. Under butyrate stress on sludge, Taihu blue algae
IntroductionTaihu Lake, the third largest freshwater lake in China, hassuffered from frequent algae blooms for several years. Everyyear more than 0.2 million tons of algae covering a quarter ofthe lake with 10 cm thickness, are harvested from Taihu Lake[1]. Algae blooms severely pollute the environment and poserisk to the safety of potable water within the area. However,due to the rapid growth of algae, the huge amount of algalbiomass can be potentially used as feedstock for sustainablebiofuels production. There are a lot of studies on biofuelsproduction from algal biomass (e.g., Chlorella vulgaris ESP6,Arthrospira platensis, and Chlamydomonas reinhardtii) [2e4].However, studies on the use of Taihu algae blooms arelimited. For instance, algae bloom freshly collected alongsideTaihu Lake (moisture content: 95%e98%; carbon, nitrogen,phosphate, and ash contents: approximately 440, 68, 6.7, and82 mg/g, respectively) pretreated with alkaline yields an energyof 1.1 kJ of hydrogen per gram of total volatile solid (TVS)through dark-fermentation [5]. In another study, acid stresson anaerobic sludge was induced using a stress mediumcontaining 12.5 g/L of acetate for 48 h with appropriateshaking. The pH was adjusted to 5.5 and the sludge waswashed twice with tap water for removing residual acidsbefore initiating the fermentation process. Acid stress onsludge also improves hydrogen energy recovery up to 1.4 kJ/gTVS[6]. Under butyrate stress on sludge, Taihu blue algae
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