3.2. Absorption spectra of the reaction product
Acetaminophen is capable of forming a yellow
chromogen nitroso derivatives by reaction with nitrite ion in an acid environment, having an
absorption maximum at 370 nm, which is not
stable and can be stabilized by the addition of
sodium hydroxide resulting in the formation of a
high stable product with a batho-chromic shift
together with a hyperchromic effect, having a
maximum at 430 nm. But our interest is in developing
a fluorometric method to enhance the sensitivity
of the method.