2.4. Wort preparation using WGBB
Four-hundred and fifty milliliter of 10 mM sodium glutamate
solution and 67.5 g of barley bran were mixed, and then the reaction was performed at 20 C for 6 h with shaking. The reaction
solution was centrifuged at 2800 g for 30 min at 4 C. The supernatant was defined as the WGBB. Wort was prepared using WGBB instead of a portion of distilled water (30, 60, or 300 mL of WGBB/
400 mL of total volume). The wort was prepared according to the
standard method of the European Brewery Convention (1987).
The malt of cultivar Ryofu harvested in Japan in 2005 was used
in the wort preparation. The wort characteristics were analyzed
according to the standard methods of the European Brewery Convention (1987) for wort clarity, wort color, boiled wort color, extract in wort, apparent attenuation limit, wort total nitrogen,
wort b-glucan, and viscosity. Each sample was prepared in
duplicate.