ting, the antibacterial activity of cotton, wool and silk fabrics with two natural dyes was found to have been enhanced significantly against S. aureus and E. coli. These fabrics are found to have a wash durability of up to 20 washes. Even using 0.5% (owf) copper sulphate mordant treatment after 15% (owf) tamarind seed coat mordant and dyed with natural dyes for all three substrates is adequately enough to obtain a good antibacterial activity against S. aureus and E. coli bacteria. The wash and light fastness properties of the dyeing on fabrics were found to be enhanced when mordanting was done. This process is promising and should be easily industrialized, popularized among the local natural dyers in order to obtain an ecofriendly product with value added properties