2.3. Determination of antifungal activity
Antifungal activity of chitosan nanoparticles (100 g) against
some phytopathogenic fungi viz., Fusarium oxysporum f.sp. ciceri,
Pyricularia grisea, Alternaria solani was evaluated by disc diffusion
method, under in vitro condition. Experiments were done in triplicate
and repeated twice.% of inhibition was calculated using the
formula: