The present study developed a carrier based HF-LPME method for the analysis of trace amounts of nine polar antibiotics in complex matrices such as milk, honey, fish, and the liver and muscles of lamb and chicken. The proposed method provides an interesting alternative to traditional sample preparation techni- ques for TCs and QNs. Compared to the traditional methods, this method exhibits very low limits of detection with higher pre- concentration factors. It has a good precision with a wide dynamic linear range. The extraction setup is simple and, due to the low cost of the hollow fiber, it can be discarded after each extraction to avoid cross-contamination.