The microalgal biomass was submitted to two conditions of acid
hydrolysis with H2SO4 1N for 60 min and H2SO4 2N for 30 min. Both
were performed at 121 C with a solid to liquid ratio of 1:7.5. After
neutralization with NaOH, the hydrolyzates were sterilized through
filtration (0.2 mm, Pall Life Sciences, USA) and kept at 4 C.