Our study required extraction of DNA from many samples and the
DNA quality had to be high enough to allow for PCR-based analysis
such as SSR marker based genetic characterization (Table 3). We
established a method that is simple, safe and fast compared to the
CTAB method which uses hazardous chemicals, is more time consuming
and significantly expensive (Doyle and Doyle, 1987). When using the
CTAB method it takes at least 6 h to complete a DNA extraction but
our method takes 10 min. In comparison to the CTAB method, our
protocol is safe enough to be performed on the lab bench in any laboratory
without requiring the use of a chemical hood. Commercially available
kits are expensive and therefore not an alternative for laboratories
with limited resources.